Doctorates' Information System

     Prabuddha Dey

Ph.D. fromIndian Institute Of Technology kharagpur - Advance technology development centre
Supervisor(s)  Dr. Mrinal K Maiti.Assistant Professor
Ph.D. status  Joined in 2009 :: In progress
AddressAdvanced laboratory for plant genetic engineering,IIT Kharagpur
Phone9475256719
Emailprabuddhamicro27@gmail.com
Formal Education
Exam / Degree Board / UnivBranchYear
M.PhilAnnamalai universityMicrobiology2007
M.Sc.kalyani universityMicrobiology2003
B.Sc.Vidyasagar UniversityBotany2001

Research Areas
  • Regulation of lipid Biosynthesis
  • Triacylglycerol synyhesis in yeast and Fungal system
  • Molecular biology and Biochemistry
  • Biofuel
  • Process optimization

Skills
  • Molecular Biology
  • Routine molecular biology techniques applied to bacteria, yeast, fungus and plant
  • rDNA technology
  • qPCR assays (SYBR-green based relative and absolute quantification of gene expression)
  • Radioactive and non-radioactive based hybridization assays with sufficient knowledge in biosafety
  • RACE (Rapid Amplification of cDNA ends) and Construction of Sub-genomic library for isol
  • Protein Biochemistry
  • Over-expression of recombinant proteins in E. coli and yeast (Saccharomyces cerevisiae, Pichia pas
  • Affinity Purification of recombinant protein (His-tagged) expressed in bacteria and yeast system
  • Purification of native protein both intracellular and extracellular from yeast and fungus by size-
  • Purification and fractionation of protein samples by Amicon-Ultra centrifugation system
  • Purification of peptide from biological samples by RP-HPLC system.
  • Native and denaturing PAGE, Detection and Quantification of proteins by Western blotting and ELISA
  • Cell Biology
  • Isolation of lipid bodies from yeast
  • Localization study of protein in yeast
  • Microbiology
  • General microbiology techniques of screening, isolation and maintainace of culture of aerobic and
  • Screenig, isolation and characterization of high lipid accumulating microbes (yeast and fungus) fr
  • Preperation of Winogradsky column in laboratory
  • Study of recycling of phosphorus and sulfide in nutrients of bacteria
  • Study of growth rate of bacteria
  • Bioinformatics
  • Conversant with Jellyfish version 3.3.1 and Sequencher 4.7 software package for nucleic acid and p
  • Proficient in operating the following softwares CLUSTAL W, BLAST, PlantCARE, PLACE, SWISS MODEL,
  • Computer knowledge
  • Proficient in Microsoft office tools, Adobe Photoshop CS, Windows 98/2000/2003/XP/2007
  • Instrumentation
  • Gas chromatography (GC) Gas chromatography-mass spectrometry (GC-MS) HPLC, MALDI-TOF
  • Fluorescence Activated Cell Sorting, Protein electro-elution system
  • Bioanalyzer, Nanodrop quantification system

Experience
  • Six years of research experience in the field of lipid synthesis of yeast, fungus and plant system

Teaching Experience
  • Microbiology(L) at R.N.L.Khan Womens College (NAAC accrediated A grade college) of Vidyasaar University 2004-2007 (3 terms)
  • Biotechnology(P) at Department of Biotechnology, IIT kharagpur 2010-2012 (1 terms)

Fellowships / Scholarships
  • DBT Junior and Senior research fellow, Govt. of India, 2008-2011
  • Institute fellow, IIT Kharagpur, 2011-2014
  • DST International travel grant, Govt. of India, 2012

Papers Published in Journals
  • Functional characterization of two structurally novel diacylglycerol acyltransferase2 isozymes responsible for the enhanced production of stearate-rich storage lipid in Candida tropicalis SY005 by Prabuddha Dey, Monami Chakraborty, Maulik R. Kamdar, Mrinal K. Maiti PLoS One, 9(4), e94472 (2014)
  • Enhancement of lipid productivity in oleaginous Colletotrichum fungus through genetic transformation using the yeast CtDGAT2b gene coupled with model-based optimization of culture condition by Prabuddha Dey, Mrinal K. Maiti PloS One under, revision (2014)
  • Molecular characterization of a novel isolate of Candida tropicalis for enhanced lipid production by Prabuddha Dey, Mrinal Kumar Maiti Journal of Applied Microbiology 114, 1357-1368 (2013)
  • Comparative lipid profiling of two endophytic fungal isolates Colletotrichum sp. and Alternaria sp. having potential utilities as biodiesel feedstock by Prabuddha Dey, Joydeep Banerjee, Mrinal K. Maiti Bioresource Technology 102, 5815-5823 (2011)
  • Identification of an extracellular antifungal protein from the endophytic fungus Colletotrichum sp. DM06 by Prabuddha Dey, Maulik R. Kamdar, Santi M. Mandal Mrinal K. Maiti Protein and Peptide letters 20(2), 173-179 (2013)
  • The attack of the phytopathogens and the trumpet solo Identification f a novel plant antifungal peptide with distinct fold and disulfide bond pattern by Santi M. Mandal, William F. Porto, Prabuddha Dey, Mrinal K. Maiti , Ananta K. Ghosh, OctavioL.Franco Biochimie 95(10), 39-48 (2013)
  • Production of nutritionally desirable fatty acids in seed oil of Indian mustard Brassica juncea L.) by metabolic engineering by Surajit Bhattacharya, Saheli Sinha, Prabuddha Dey, Natasha Das, Mrinal K. Maiti Phytochemistry Review 11, 197-209 (2012)
  • Transgenically expressed rice germin-like protein1 in tobacco causes hyper-accumulation of H2O2 and reinforcement of the cell wall components by Joydeep Banerjee, Natasha Das, Prabuddha Dey, Mrinal K. Maiti Biochemical and Biophysical Research Communications 402, 637-643 (2010)
  • Effect of organic forms of phosphorus and variable concentrations of sulfide on metabolic generation of soluble-reactive phosphate by sulfur chemolithotrophs a laboratory study by Himadri Guhathakurta, Raka Biswas, Prabuddha Dey, Piyali Gajendra Mahapatra and Bipasha Mondal The ISME Journal 1,545-550 (2007)
  • Genetic transformation of endophytic fungus Colletotrichum sp. DM06 for enhanced production of storage lipid by Prabuddha Dey, Monami Chakraborty, Mrinal K. Maiti 15th International Biotechnology Symposium (IBS 2012), 16-21 September, Daegu, South Korea, 2012 16-21 September, Daegu (2012)

Indian Institute of Technology, Kharagpur-721 302, INDIA
Telephone Number +91-3222-255221 | FAX : +91-3222-255303