Doctorates' Information System

     Nandana Bhardwaj

Ph.D. fromIndian Institute of Technology, Kharagpur - Biotechnology
Supervisor(s)  Professor S. C. Kundu
Ph.D. status  Joined in 2007 :: Completed in 2011
AddressRLB Hall, Room No. A-120, IIT Kharagpur
Formal Education
Exam / Degree Board / UnivBranchYear
M.Sc.Himachal Pradesh University, ShimlaBiotechnology2006
B.Sc.Patna University (Patna Womens College)Industrial Microbiology2003

Research Areas
  • Biomaterials
  • Tissue Engineering (Cartilage)
  • Stem Cell Biology
  • Bioengineering

  • Instrumentation - SEM, FTIR, XRD, FACS, Microscopy(Confocal, Fluorescence)
  • Gel electrophoresis (Agarose and PAGE)
  • Histology and Immunohistochemical (IHC) techniques
  • Chromatographic techniques (TLC, Ion exchange, Gel filtration, Affinity)
  • Primary cell isolation and culture, cell culture techniques, Handling of cell lines
  • Spectroscopic techniques
  • Computer and software skills
  • 3D scaffolds, films and nanofibers fabrication
  • Enzymological and Immunological techniques (ELISA, Immunoelectrophoresis)
  • Microbiological techniques (Staining, MBRT, MIC and IMVIC test, CFU determination)
  • Biochemical studies (Estimation of carbohydrate, protein, collagen, GAG, DNA, RNA)
  • Molecular biology techniques (DNA, plasmid and RNA isolation, Real time PCR, cloning, expression)

  • Qualified All India Entrance for Masters in Biotechnology conducted by JNU, New Delhi,2004
  • Awarded DBT fellowship for Masters (2004-2006)
  • Awarded DBT- JRF (2007-2009)
  • Awarded DBT-SRF (2009- 2011)
  • Qualified GATE, 2007 (98.26 percentile score and AIR 195)
  • Qualified CSIR-NET for Lecturership (Dec, 2006)
  • Awarded DST travel grant for attending International Conference of Tissue Engineering and Regerative Medicine International Society meeting Singapore (August, 3-5 August ,2011)
  • Nature India special highlight for our research work titled Scaffolds to grow cartilage (doi10.1038/nindia.2011.138).
  • Nature India special research highlight for our research work titled Scaffolds for stem cells to grow cartilage doi10.1038/nindia.2012.43).

  • Visiting Grad student University of California, San Diego, USA (3 months)
  • Visiting Grad student in Tufts university, USA (September, 2009)
  • 3 months research experience with cartilage tissue engineering using 3-D silk scaffolds at University of California, San Diego, USA
  • Two months winter training at IMTECH, Chandigarh on topic entitled (Purification, cloning and expression of vitreoscilla haemoglobin gene
  • One year in house project on- Optimization of production and reaction conditions of pectin lyase of B. fulva (MTCC-505) at HPU, Shimla
  • Assisted in event management of Bioinformatics Workshop. Genomics, Proteomics and Metabolomics, IIT Kharagpur (2007-2010)
  • Assisted in event management of International Conference on Biomaterials and Tissue Engineering for Biotechnological Applications (BTEB 2008), IIT Kharagpur, India, November 22-24
  • Four year 4 months (June 2007-October, 2011) Ph. D research experience as JRF and SRF in the field of Biomaterials and Tissue Engineering.

Fellowships / Scholarships
  • DBT fellowship for Maters in Biotechnology (2004-2006)
  • DBT-Junior Research Fellowship (2007-2009)
  • DBT-Senior Research Fellowship (2009 - 2011)
  • Fellowship supported from IUSSTF (Indo-US forum, New Delhi), (3 months, 2009)

Papers Published in Journals
  • Electrospinning A fascinating fiber fabrication technique. by Nandana Bhardwaj and Subhas C. Kundu Biotechnology Advances 28 (3), 325-347 (2010)
  • Silk fibroin protein and chitosan polyelectrolyte complex porous scaffolds for tissue engineering applications by Nandana Bhardwaj and Subhas C. Kundu Carbohydrate polymers 85 (2), 325-333. (2011)
  • Potential of 3-D tissue constructs engineered from bovine chondrocyte / silk fibroin-chitosan for in vitro cartilage tissue engineering. by Nandana Bhardwaj, Quynhhoa T Nguyen, Albert C Chen, David L Kaplan, Robert L Sah, Subhas C Kundu Biomaterials 32 (25), 5773-5781 (2011)
  • Freeze-gelled silk fibroin protein scaffolds for potential applications in soft tissue engineering by Nandana Bhardwaj, Sagar Chakraborty and Subhas C. Kundu, International Journal of Biological Macromolecules 49, 260-267 (2011)
  • Biotechnology of the silk proteins challenges, approaches and applications by Sunita Nayak, Nandana Bhardwaj, Sarmistha Talukdar, Banani Kundu, Subia Bano and S C Kundu Biotechnology in Biopolymers, iSmithers-Rapra Publication, UK accepted (2011)
  • Chondrogenic differentiation of rat MSCs on porous scaffolds of silk fibroin/chitosan blends. by Bhardwaj N and Kundu SC. Biomaterials 33 (10), 2848-2857 (2012)
  • Non-mulberry silk biopolymers. by Kundu SC, Kundu B, Talukdar S, Bano S, Nayak S, Kundu J, Mandal BB, Bhardwaj N, Botlagunta M, Dash BC, Acharya C, and Ghosh AK Biopolymers doi10.1002/ bip.220 (2012)

Papers Presented at Conferences
  • Non- mulberry Silk Proteins Advanced Natural Biomaterial for Tissue Engineering and Drug Delivery by S. C. Kundu, C. Acharya, R. Dash, B. B Mandal, Kundu, J. N. Bhardwaj, S. Nayak, S. Talukdar, M. Dewan and B. Kundu Second TERMIS World Congress August 31-September 03, Seoul, Republic of Korea ---- (2009)
  • Preparation, characterization and in vitro evaluation of silk fibroin/chitosan blended 3-d scaffolds by N. Bhardwaj and S. C. Kundu International conference on Biomaterials and Tissue Engineering for Biotechnological Applications (BTEB 2008), IIT Kharagpur, India, November 22-24) 39 (2008)
  • Silk Proteins as Biomaterial for Tissue Engineering and Regenerative Medicine by Nandana Bhardwaj, Sunita Nayak and Subhas C. Kundu, International workshop on New Visions for Biomaterials and Regenerative Medicine, 16-17 March, 2011, Sree Chitra Tirunal Institute for Medical Sciences and Technology,Thiruvananthapuram, Kerala 15-19 (2011)
  • SILK FIBROIN/CHITOSAN POLYELECTROLYTE COMPLEX 3D TISSUE CONSTRUCTS FOR CARTILAGE TISSUE ENGINEERING by Nandana Bhardwaj, Quynhhoa T Nguyen, Albert C Chen, David L Kaplan, Robert L Sah, Subhas C Kundu Tissue Engineering and Regenerative Medicine International Society Meeting (TERMIS-AP), 3-5 August, 2011, Singapore 49 (2011)

Indian Institute of Technology, Kharagpur-721 302, INDIA
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