Doctorates' Information System

     Sangeeta Choudhary

Ph.D. fromIndian Institute of Technology - Biotechnology
Supervisor(s)  Dr Pinaki Sar
Ph.D. status  Joined in 2005 :: In progress
Address19/306, Indira Nagar, Lucknow
Phone9434958828
Emailsangeeta25@gmail.com
Formal Education
Exam / Degree Board / UnivBranchYear
10Council for Indian School Certificate Examinations (ICSE), New DelhiEnglish, Hindi, Mathematics, History Civics and Geography, Science, Economics1994
12Council for Indian School Certificate Examination, New DelhiEnglish, Hindi, Physics, Chemistry and Biology1996
B.Sc.Banaras Hindu University,VaranasiZoology (Hons), Botany, Chemistry2000
M.Sc.Banaras Hindu University,VaranasiZoology (Specialization, Molecular, applied and clinical genetics)2002

Research Areas
  • Environmental Microbiology and Biotechnology
  • Microbial bioremediation of heavy metals and radionuclides
  • Bioinformatics

Skills
  • Microbiological techniques,Enrichment, isolation, identification and characterization of bacteria
  • Biochemical and 16s rRNA based identification of bacteria
  • PCR-based molecular phylogenic analyses and identification of microbes using ribosomal RNA genes
  • Routine molecular biology protocols for extraction of plasmid / genomic DNA, RNA from microorganisms
  • PCR, Gel purification, transformation, cloning, sequencing and sequence analysis
  • Agarose gel electrphoresis, PAGE, Gel documenation,
  • Real Time PCR (Expression analysis, absolute and relative quantification)
  • Transmission and Scanning electron microscope, Atomic force microscopy (AFM), EDX
  • Whole cell, periplasmic, cytoplasmic and membrane protein isolation from bacteria
  • UV-Visible, X-ray powder diffractometer (XRD), Energy dispersive X-ray, Infrared (FTIR) spectroscope
  • Atomic absorption spectrophotometer (AAS), ICP-MS
  • Primer designing in Primer 3, biotools, ABI PRIMER express
  • Bioinformatics , NCBI tools, Ribosomal Database Project, bioedit, chromas, Expasy tools
  • Expertise in Phylogenetic analysis in various phylogenetic tool packages

Awards
  • GATE, 2002, 98.02 PERCENTILE, AIR 54
  • CSIR-UGC, NET JRF in Life Science

Experience
  • Assisted in hands-on training of the workshop on Bioinformatics in Genomics and Proteomics, September 22-23.2006, Department of Biotechnology, IIT ,Kharagpur
  • Attended workshop on Flow cytometry and its Research Applications on BD FACSCalibur, December18-19,2006, IIT ,Kharagpur

Fellowships / Scholarships
  • CSIR-UGC, NET, JRF and SRF

Papers Published in Journals
  • Characterization of a metal resistant Pseudomonas sp isolated from uranium mine for its potential in heavy metal (Ni, Co, Cu, and Cd) sequestration. by Sangeeta Choudhary and Pinaki Sar Bioresource Technology 100, 2482 to 2492 (2009)

Papers Presented at Conferences
  • Real-time PCR based detection of a Pseudomonas strain J007, isolated from uranium contaminated site of Jaduguda, India Potential in Monitored Natural Attenuation (MNA) by Sangeeta Choudhary and Pinaki Sar International Conference on Emerging Trends in Biotechnology (ETBT-2009), Banaras Hindu University, Varanasi, India XXXX (2009)

Indian Institute of Technology, Kharagpur-721 302, INDIA
Telephone Number +91-3222-255221 | FAX : +91-3222-255303